Journal: Bioactive Materials

Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

doi: 10.1016/j.bioactmat.2026.01.005

Figure Lengend Snippet: The biocompatibility of Ti-OH-ePV. a) Schematic illustration of the co-culture model; b) Viability of L929 cells after 24 h co-culture with Ti, Ti-OH, and Ti-OH-ePV samples; c) Live/dead fluorescence images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 200 μm); d) Cytoskeletal staining images of L929 cells in Ti, Ti-OH, and Ti-OH-ePV groups (scale bar: 100 μm); e) Hemolysis test results and f) quantitative hemolysis ratios of Triton X-100, PBS, Ti, Ti-OH, and Ti-OH-ePV groups; g) Immunohistochemical staining images of IL-4, IL-10, CD68, and IL-1β in rat subcutaneous tissues 7 days post-implantation (scale bar: 100 μm); h) Quantitative analysis of IL-4 and IL-10 expression; i) Quantitative analysis of CD68 and IL-1β expression; n = 3; ns = no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: Standard fibroblast cell line L929 fibroblasts and mouse macrophages (Raw264.7) were obtained from Jiangsu Keygen Biotech Co., Ltd. (China).

Techniques: Co-Culture Assay, Fluorescence, Staining, Immunohistochemical staining, Expressing

Journal: Bioactive Materials

Article Title: Bioactive-coated porous anastomotic staples enhance anastomotic healing

doi: 10.1016/j.bioactmat.2026.01.005

Figure Lengend Snippet: Evaluation of cell adhesion, proliferation, and pro-angiogenic potential of different samples. a) SEM images of Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 1 μm); b) Fluorescent images of L929 cells on Ti, Ti-OH, Ti-OH-ePV-3d, and Ti-OH-ePV-7d after co-culturing 72 h (scale bar: 200 μm); c) Fluorescent images of Matrigel tube formation by HUVECs treated with Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d (scale bar: 200 μm); e) Scratch wound migration assay of HUVECs in the Ti, Ti-OH, Ti-OH-ePDA, Ti-OH-ePV-3d, and Ti-OH-ePV-7d groups (scale bar: 100 μm).

Article Snippet: Standard fibroblast cell line L929 fibroblasts and mouse macrophages (Raw264.7) were obtained from Jiangsu Keygen Biotech Co., Ltd. (China).

Techniques: Migration

Journal: ACS Omega

Article Title: Comparative Study of Chitosan-Pyrophosphate and Magnesium Hydroxide-Alginate Hybrid Nanoparticles: Physicochemical Properties and Cytocompatibility toward Vascular Calcification Applications

doi: 10.1021/acsomega.5c12883

Figure Lengend Snippet: (a) Cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-Mg­(OH) 2 -Alg at different concentrations. * p < 0.05 vs control; (b) cytotoxicity analysis of L929 cells after 24 h of exposure to NPs-CS-PPi at different concentrations. * p < 0.05 vs control.

Article Snippet: The murine fibroblast cell line L929 (ATCC NCTC clone 929 [L cell, CCL-1]) was used for cytotoxicity assays.

Techniques: Control

Journal: Bioactive Materials

Article Title: Degradation behavior and biological properties of a novel biodegradable zinc alloy biliary stent

doi: 10.1016/j.bioactmat.2025.12.022

Figure Lengend Snippet: Cytocompatibility of Zn alloys. A-C : The viability of L929 cells after 1, 3, and 5 days of treatment with Zn alloy extracts, respectively. D-F : The viability of RAW264.7 cells after 1, 3 and, 5 days of treatment with Zn alloy extracts, respectively. G: Cell viability and statistical analysis ( H–I ) of L929 cells after 24h and 72 h of direct contact with Zn alloy discs (scale bar: 200 μm). * p < 0.05.

Article Snippet: Penicillin-Streptomycin Solution, RAW264.7 Murine Macrophages, and L929 Murine Fibroblasts were purchased from Procell (China).

Techniques:

Journal: Bioactive Materials

Article Title: Degradation behavior and biological properties of a novel biodegradable zinc alloy biliary stent

doi: 10.1016/j.bioactmat.2025.12.022

Figure Lengend Snippet: The content of Caspase-3 and ROS in cells treated with Zn alloy extract. A–B: The content of Caspase-3 ( A ) ROS ( B ) and in L929 cells treated with different Zn alloy extract. C-D: The content of Caspase-3 ( C ) ROS ( D ) and in RAW264.7 cells treated with Zn alloy extract. Magnification: 40 × , Scale bar: 10 μm.

Article Snippet: Penicillin-Streptomycin Solution, RAW264.7 Murine Macrophages, and L929 Murine Fibroblasts were purchased from Procell (China).

Techniques: